Cloning Vectors

Cloning Vectors: Vectors are DNA molecules that can carry foreign DNA into another cell and also replicate it. Also known as vehicle DNA. Essential features of a cloning vector: Origin of replication: ORI is a specific sequence on a DNA molecule from where replication starts. It will also replicate the …

Restriction Endonuclease

Restriction Endonuclease: Discovered by Werner Arber, Daniel Nathans, and Hamilton Smith. They are known as “molecular scissors” in genetic engineering. They are capable of breaking DNA at specific sites called the restricted sites. These restricted sites have Palindromic sequences. Palindromic sequences are sequences that have the same meaning when read …